Quantitative evaluation of the dynamic activity of HeLa cells in different viability states using dynamic full-field optical coherence microscopy

This research article demonstrates a method to quantitatively analyze the dynamic activities of HeLa cells using Dynamic Full-Field Optical Coherence Microscopy (DFFOCM). By continuously monitoring the frequency and magnitude of intracellular scatterer movements, the researchers were able to clearly distinguish between viable and non-viable cells without the need for staining or chemical markers. The study suggests that DFFOCM is a promising, non-invasive alternative to conventional histological methods for assessing cell viability and detecting cell death processes like apoptosis and necrosis in real-time.